Download e-book for iPad: Next-Generation Sequencing Data Analysis by Xinkun Wang
By Xinkun Wang
A useful consultant to the hugely Dynamic sector of vastly Parallel Sequencing
The improvement of genome and transcriptome sequencing applied sciences has resulted in a paradigm shift in existence technology learn and ailment analysis and prevention. Scientists are actually capable of see how human illnesses and phenotypic adjustments are attached to DNA mutation, polymorphism, genome constitution, and epigenomic abnormality. Next-Generation Sequencing info Analysis exhibits how next-generation sequencing (NGS) applied sciences are utilized to remodel approximately all elements of organic study.
The ebook walks readers in the course of the a number of phases of NGS info iteration and research in an easy-to-follow type. It covers each step in each one degree, from the strategy planning stage of experimental layout, pattern processing, sequencing technique formula, the early degree of base calling, reads caliber cost and information preprocessing to the intermediate degree of mapping reads to a reference genome and normalization to extra complex levels particular to every software. All significant purposes of NGS are coated, including:
- RNA-seq: mRNA-seq and small RNA-seq
- Genotyping and variation discovery via genome re-sequencing
- De novo genome assembly
- ChIP-seq to check DNA–protein interaction
- Methylated DNA sequencing on epigenetic regulation
- Metagenome research via neighborhood genome shotgun sequencing
Before detailing the analytic steps for every of those purposes, the booklet offers the bits and bobs of the main normal NGS structures, with side-by-side comparisons of key technical points. This is helping practitioners make a decision which platform to exploit for a specific undertaking. The ebook additionally deals a point of view at the improvement of DNA sequencing applied sciences, from Sanger to future-generation sequencing applied sciences.
The booklet discusses recommendations and rules that underlie each one analytic step, in addition to software program instruments for implementation. It highlights key good points of the instruments whereas omitting tedious information to supply an easy-to-follow advisor for practitioners in existence sciences, bioinformatics, and biostatistics. additionally, references to exact descriptions of the instruments are given for extra examining if wanted. The accompanying site for the publication offers step by step, real-world examples of the way to use the instruments lined within the textual content to analyze initiatives. the entire instruments are freely to be had to educational users.